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stat3 124h6 cell signaling technology  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stat3 124h6 cell signaling technology
    Stat3 124h6 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/124h6+cell+signaling/Phospho-Stat3+(Tyr705)+XP+Rabbit+mAb/pm41482360-98-40-42
    Average 98 stars, based on 6117 article reviews
    stat3 124h6 cell signaling technology - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Western Blot:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Enzyme-linked Immunosorbent Assay:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    In Vitro:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    In Vivo:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Control:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Recombinant:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Staining:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Purification:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Protease Inhibitor:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025

    Transfection:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays TransnetYX Genotyping Service TransnetYX https://www.transnetyx.com/ BD Cytofix/Cytoperm Fixation/ Permeabilization Kit BD Biosciences Cat# 554714 Deposited data Xena Functional Genomics Explorer Database for TCGA Breast Cancer Gene Analysis Goldman et al.80 https://xenabrowser.net/datapages/? dataset=TCGA.BRCA.sampleMap TIMER2.0 Database for TCGA Breast Cancer Gene Analysis Li et al.81 http://timer.cistrome.org/ KM Plotter Survival Analysis Gyorffy82 https://kmplot.com/analysis/index.php? p=service Experimental models: Cell lines Human: MDA-MB-231 Laboratory of Pepper Schedin RRID: CVCL_0062 Mouse: 66cL4 cell line Laboratory of Heide Ford RRID: CVCL_9721 Mouse: 66cL4-DDK Elder et al.20 N/A Mouse: 66cL4-DDK-SEMA7A Elder et al.20 N/A Mouse: 66cL4-SEMA7A knockdown This Paper N/A Mouse: E0771 cell line CH3 Biosystems Cat# 940001; RRID: CVCL_GR23 Mouse: E0771-DDK Elder et al.20 N/A Mouse: E0771-DDK-SEMA7A Elder et al.20 N/A Experimental models: Organisms/strains Mouse: BALB/cAnNCrl Charles River RRID: IMSR_CRL:028 Mouse: C57BL/6NCrl Charles River RRID: IMSR_CRL:027 Mouse: Sema7a knockout: Sema7atm1Alk/J Laboratory of Alex Kolodkin (Now available from Jackson Labs) RRID: IMSR_JAX005128 Mouse: Sema7a heterozygous: Sema7atm1Alk/J x C57BL/6 This Paper N/A Recombinant DNA Plasmid: Myc-DDK Origene Cat# PS100001 Plasmid: Myc-DDK-Sema7a Origene Cat# MR209876 AACN: NM_011352 Plasmid: Sema7a shRNA knockdown CU Functional Genomics Shared Resource RRID: SCR_021987 Software and algorithms BioTek Gen5 v3.11 Agilent https://www.agilent.com/en/product/ microplate-instrumentation/microplateinstrumentation-control-analysis-software/ imager-reader-control-analysis-software/ biotek-gen5-software-for-detection1623227 FlowJo Software v10 BD Biosciences https://www.flowjo.com/ GraphPad Prism v10 Dotmatics https://www.graphpad.com/ ImageJ Schneider et al.110 https://imagej.net/ij/ IncuCyte Zoom 2020A software Essen Bioscience https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software ZE5-YETI Everest software Bio-Rad https://www.bio-rad.com/en-us/sku/ 12009761-everest-software-developer-kitze5-automation?ID=12009761 20 Cell Reports 44, 115676, May 27, 2025



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    Image Search Results


    FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical PI3K/Akt/FOXO1 and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total FOXO1 protein (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).

    Journal: The FASEB Journal

    Article Title: KCNB1‐Leptin receptor complexes couple electric and endocrine function in the melanocortin neurons of the hypothalamus

    doi: 10.1096/fj.202401931r

    Figure Lengend Snippet: FIGURE 6 Canonical and non-canonical leptin pathways are altered in NULL. (A) Graphical illustration of LepR signaling. Canonical (JAK2/STAT3) and non-canonical PI3K/Akt/FOXO1 and ERK pathways are indicated. (B) Representative Western blot and densitometric quantification of the long isoform leptin receptor (LepR) in hypothalamic lysates of WT and NULL mice. (C) Representative Western blot of STAT3 phosphorylated at Y705 (pSTAT3) and total STAT3 protein (STAT3) in hypothalamic lysates of WT and NULL mice in absence/presence of leptin. (D) Densitometric quantifications of the fraction of pSTAT3/STAP calculated from Westerns as that in (C). (E) Representative Western blot of phosphorylated FOXO1 at S256 (pFOXO1) and total FOXO1 protein (FOXO1) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (F) Densitometric quantifications of pFOXO1/FOXO1 calculated from Westerns as those in (E). (G) ELISA quantifications of phosphorylated Akt at S473 (pAkt) over total Akt protein (pAkt/Akt) in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. (H) Representative Western blots of phosphorylated ERK1/2 at T202/T204 (pERK) and total ERK1/2 (ERK) protein in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin and corresponding densitometric quantifications of the fraction of phosphorylated ERK1/2 over total ERK1/2 (pERK/ERK). (I) ELISA quantifications of αMSH in hypothalamic lysates of WT and NULL mice in the absence/presence of leptin. Western blot loading controls were actin and Bradford colorimetric assay. Animals were injected with either vehicle or 1.0 mg/kg body weight leptin and sacrificed 90 min later. *p < .05 (ANOVA with Tukey's post hoc).

    Article Snippet: Reagent or resource Source Identifier Antibodies POMC (D3R1U) Rabbit mAb Cell Signaling Technology #23499 c- Fos (9F6) Rabbit mAb Cell Signaling Technology #2250 Stat3 (124H6) Mouse mAb Cell Signaling Technology #9139 Phospho- Stat3 (Tyr705) (M9C6) Mouse mAb Cell Signaling Technology #4113 FoxO1 (C29H4) Rabbit mAb Cell Signaling Technology #2880 Phospho- FoxO1 (Ser256) (E1F7T) Rabbit mAb Cell Signaling Technology #84192 p44/42 MAP kinase (phosphorylated Erk1/2) Cell Signaling Technology Cat# 9101 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb Cell Signaling Technology Cat# 4695 Integrin beta- 5 (D24A5) Rabbit mAb Cell Signaling Technology Cat# 3629 Integrin beta- 1 antibody Cell Signaling Technology Cat #4706 Alpha MSH polyclonal rabbit Bioss Antibodies bs- 1848R AgRP polyclonal antibody Thermo Fisher PA5- 118934 Leptin receptor polyclonal antibody Thermo Fisher PA1- 053 Leptin receptor recombinant Rabbit monoclonal antibody Thermo Fisher MA5- 32685 (NIH), Grant/Award Number: R01AG060919; National Science Foundation (NSF), Grant/Award Number: 2030348 upon leptin stimulation.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, Injection